Immunohistochemical localization of integrin αVβ3 in cryostat cross sections of bovine endometrial tissue during the estrous cycle (A and C, day 18 cyclic, B, day 16 cyclic) and at different stages of pregnancy (D, day 16 pregnant, E, day 18 pregnant, F, day 21 pregnant, G, day 24 pregnant, H, day 30 pregnant). Positive antibody reactivity (shown as brown) was strongest in intercaruncular (ICAR) subepithelial stroma (SES) as indicated by the arrow, except at day 16 of the estrous cycle (B) as reported previously 14. Note the low reactivity in the caruncles (CAR), glands (G), superficial gland (SG) and trophoblast (T). A section treated with mouse IgG instead of primary antibody (negative control) is shown in panel A. Bar = 50 μm.