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Table 1 Sequences of oligonucleotide primers used for PCR amplification

From: Stimulation of TM3 Leydig cell proliferation via GABAA receptors: A new role for testicular GABA

Target GenBank accession no. Length (bp) Primer sequence
GAD67 NM_008077 393 5'-CTTCTTCCGGATGGTCATCTC-3'
    5'-ACGAGCAACATGCTATGGTCT-3'
GAD65 NM_008078 372 5'-GATATGGTTGGATTAGCAGC-3'
    5'-CATTTTCCCTCTCTCATCAC-3'
VGAT NM_009508 300 5'-CCTGTACGAGGAGAACGAAG-3'
    5'-AGCAGACTGAACTTGGACAC-3'
GABAA α1 NM_010250 231 5'-CTACAGCAACCAGCTATACCC-3'
    5'-GCTCTCTGTTTAAATACGTGG-3
GABAA α2 NM_008066 282 5'-AAGGCTCCGTCATGATACAG-3'
    5'-ACTAACCCCTAATACAGGC-3'
GABAA α3 NM_008067 418 5'-GACTTGCTTGGTCATGTTGTTGGG-3'
    5'-CAGAGGCCCTGGAGATGAAGAAGA-3'
GABAA β1 NM_008069 540 5'-ATGATGCATCTGCAGCCA-3'
    5'-TGGAGTTCACGTCAGTCA-3'
GABAA β2 NM_008070 402 5'-GCATGTATGTCTGCAGGA-3'
    5'-CTGACACCTACTTCCTGA-3'
GABAA β3 NM_008071 224 5'-AGCCAAGGCCAAGAATGATCG-3'
    5'-TGCTTCTGTCTCCCATGTACC-3'
GABAA γ1 X55272 191 5'-TTTCTTACGTGACAGCAATGG-3'
    5'-CATGGGAATGAGAGTGGATCC-3'
GABAA γ2 NM_008073 351 5'-GCAATGGATCTCTTTGTA-3'
    5'-GTCCATTTTGGCAATGCG-3'
GABAA γ3 NM_008074 251 5'-TGTCGAAAGCCAACCATCAGG-3'
    5'-GACTTGCACTCCTCATAGCAG-3'
GABAB R1 NM_019439 350 5'-ATGTGGTAACCATCCAGC-3'
    5'-AGAAGATCGGCTACTACG-3'
GABAB R2 XM_194138 354 5'-CATCATCTTCTGTAGCAC-3'
    5'-TCTGTGAAGTTGCCCAAG-3'