Table 1 Sequences of oligonucleotide primers used for PCR amplification
From: Stimulation of TM3 Leydig cell proliferation via GABAA receptors: A new role for testicular GABA
| Target | GenBank accession no. | Length (bp) | Primer sequence |
|---|---|---|---|
| GAD67 | NM_008077 | 393 | 5'-CTTCTTCCGGATGGTCATCTC-3' |
| 5'-ACGAGCAACATGCTATGGTCT-3' | |||
| GAD65 | NM_008078 | 372 | 5'-GATATGGTTGGATTAGCAGC-3' |
| 5'-CATTTTCCCTCTCTCATCAC-3' | |||
| VGAT | NM_009508 | 300 | 5'-CCTGTACGAGGAGAACGAAG-3' |
| 5'-AGCAGACTGAACTTGGACAC-3' | |||
| GABAA α1 | NM_010250 | 231 | 5'-CTACAGCAACCAGCTATACCC-3' |
| 5'-GCTCTCTGTTTAAATACGTGG-3 | |||
| GABAA α2 | NM_008066 | 282 | 5'-AAGGCTCCGTCATGATACAG-3' |
| 5'-ACTAACCCCTAATACAGGC-3' | |||
| GABAA α3 | NM_008067 | 418 | 5'-GACTTGCTTGGTCATGTTGTTGGG-3' |
| 5'-CAGAGGCCCTGGAGATGAAGAAGA-3' | |||
| GABAA β1 | NM_008069 | 540 | 5'-ATGATGCATCTGCAGCCA-3' |
| 5'-TGGAGTTCACGTCAGTCA-3' | |||
| GABAA β2 | NM_008070 | 402 | 5'-GCATGTATGTCTGCAGGA-3' |
| 5'-CTGACACCTACTTCCTGA-3' | |||
| GABAA β3 | NM_008071 | 224 | 5'-AGCCAAGGCCAAGAATGATCG-3' |
| 5'-TGCTTCTGTCTCCCATGTACC-3' | |||
| GABAA γ1 | X55272 | 191 | 5'-TTTCTTACGTGACAGCAATGG-3' |
| 5'-CATGGGAATGAGAGTGGATCC-3' | |||
| GABAA γ2 | NM_008073 | 351 | 5'-GCAATGGATCTCTTTGTA-3' |
| 5'-GTCCATTTTGGCAATGCG-3' | |||
| GABAA γ3 | NM_008074 | 251 | 5'-TGTCGAAAGCCAACCATCAGG-3' |
| 5'-GACTTGCACTCCTCATAGCAG-3' | |||
| GABAB R1 | NM_019439 | 350 | 5'-ATGTGGTAACCATCCAGC-3' |
| 5'-AGAAGATCGGCTACTACG-3' | |||
| GABAB R2 | XM_194138 | 354 | 5'-CATCATCTTCTGTAGCAC-3' |
| 5'-TCTGTGAAGTTGCCCAAG-3' |