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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: Stimulation of TM3 Leydig cell proliferation via GABAA receptors: A new role for testicular GABA

Figure 6

GAD is active in TM3 cells. GAD activity [cpm/μg protein] was measured in TM3 cells (n = 11 batches of cells) and in AtT20 cells (n = 9 batches of cells), another endocrine mouse cell line used for control purposes. TM3 cells (Co1, n = 6 batches of cells) and testicular samples of adult rats (Co2, n = 6), both heated to 95°C for 5 min, served as additional negative controls. Testicular GAD activity in TM3 cells is higher than GAD activity in AtT20 cells or in controls. Columns with different superscripts are significantly (ANOVA/Newmann-Keuls, p < 0.001) different from each other and represent means+SEMs.

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