Table 1 Details of the primary and secondary antibodies used for western immuno-blotting in this experiment
Antibody | Type of antibody | Species | Source | Final dilution |
|---|---|---|---|---|
Primary | Anti-cytochrome P450 Aromatase P450 | Mouse monoclonal | ABD Serotec, Cergy Pontoise, France | 1:200 |
Anti-Akt | Rabbit polyclonal | Cell Signalling (OZYME), Saint Quentin Yvelines, France | 1:1,000 | |
Anti-phospho-Akt1/2/3 (Ser473)-R | Rabbit polyclonal | Santa Cruz Biotechnology, Tebu-Bio, Le Perray-en_ Yvelines | 1:1,000 | |
Anti-AMPK | Rabbit polyclonal | Cell Signalling (OZYME), Saint Quentin Yvelines, France | 1/1,000 | |
Anti-phospho-AMPK Thr 172 | Rabbit polyclonal | Cell Signalling (OZYME), Saint Quentin Yvelines, France | 1:1,000 | |
Anti-Erk2 (C-14) | Rabbit polyclonal | Cell Signalling (OZYME), Saint Quentin Yvelines, France | 1:1,000 | |
Anti-phospho-ERK1/2 | Rabbit polyclonal | Cell Signalling (OZYME), Saint Quentin Yvelines, France | 1/1,000 | |
Anti-vinculin | Mouse monoclonal | Sigma Aldrich, Saint-Quentin Fallavier, France | 1:1,000 | |
Secondary | Anti-Mouse IgG | Goat polyclonal | Laboratoires Eurobio, Courtaboeuf, France | 1:10,000 |
Anti-Rabbit IgG | Goat polyclonal | Laboratoires Eurobio, Courtaboeuf, France | 1:10,000 |