Table 1 Sequence of primers used in the present study
Primer | Sequence | Purpose |
|---|---|---|
Dmrt1 I-F | 5’-TGCAGAAACCACGGCTTC-3’ | cDNA fragment PCR |
Dmrt1 I-R | 5’-GATGCCCATCTCCTCCTC-3’ | cDNA fragment PCR |
Dmrt1 II-F | 5’-GGCAGTCCCTCCAGTTACAG-3’ | cDNA fragment PCR |
Dmrt1 II-R | 5’-GGGAGGGCTGGTAAAAGTTG-3’ | cDNA fragment PCR |
Dmrt1 III –R | 5’-GRGACAYGTTRTGGCTGGAC-3’ | cDNA fragment PCR |
Sox9 I-F | 5’-GAAGGACCATCCCGACTACA-3’ | cDNA fragment PCR |
Sox9 I-R | 5’-GKGTRTACATKGGCCTCTGG-3’ | cDNA fragment PCR |
Sox9 II-F | 5’-CGAACGTGTTCGGGAACTTA-3’ | cDNA fragment PCR |
Sox9 II-R | 5’-GGCGTGGCTGTAGTAGGAGT-3’ | cDNA fragment PCR |
Dmrt1 5UTR | 5’-AGAGCCACCTGAGCGGCCATGACCC-3’ | RACE |
Dmrt1 3UTR | 5’-GCCTACTACAGCAACCTCTACAATTATCAGCAATACCA-3’ | RACE |
Sox9 5UTR I | 5’-GCACGAGGATCTCTTCCCCCTTAGTTTCTACGCATTTT-3’ | RACE |
Sox9 5UTR II | 5’-GCTCCGCGTTGTGCAGATGCGGGTACTGGT-3’ | RACE |
Sox9 3UTR | 5’-CGCATCTGCACAACGCGGAGCTCAGCAA-3’ | RACE |
Dmrt1-RT-F | 5’-CAGCCTACTACAGCAACCTCTACAAT-3’ | qRT-PCR |
Dmrt1-RT-R | 5’-TGGCTGGACAGACGGCTATC-3’ | qRT-PCR |
Sox9-RT-F | 5’-CCAGCATGGGCGAAGTG-3’ | qRT-PCR |
Sox9-RT-R | 5’-CGTCGGTGGCGTTGGA-3’ | qRT-PCR |
Ef1a-RT-F | 5’-CTTCTCAGGCTGACTGTGC-3’ | qRT-PCR |
Ef1a-RT-R | 5’-CCGCTAGCATTACCCTCC-3’ | qRT-PCR |
β-Actin-F-Control | 5’-CCATCTCCTGCTCGAAGTC-3’ | Internal control |
β-Actin-R-Control | 5’-CACTGCCCATCTACGAG-3’ | Internal control |