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Figure 8 | Reproductive Biology and Endocrinology

Figure 8

From: Molecular cloning and expression analysis of dmrt1 and sox9 during gonad development and male reproductive cycle in the lambari fish, Astyanax altiparanae

Figure 8

Testes of A. altiparanae in different phases of annual reproductive cycle. (A) Early GE development: continuous GE with cysts in different phases of spermatogenesis and low amount of free germ cell in the lumen (arrow); (B) Mid GE development: discontinuous GE, with some spermatogonia (arrowhead) and several free germ cells in the tubule lumen (arrow). (C) Late GE development: where discontinuous GE (*) could be found with a great amount of free cells immersed in the secretion found in the tubule lumen (arrow). (D) Regressed phase: high amount of spermatogonia (arrowhead) and Sertoli cells in the GE. Relative mRNA expression levels of dmrt1 (E) and sox9 (F) during different testes maturation phases. Values are expressed as arbitrary units of both mRNA levels normalized against the expression levels of ef1a amplified from the same template and relative to the expression observed during early GE development. Scale bar = 50 μm.

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