Figure 6

Gonad development of A. altiparanae (A). Longitudinal view of a 5 dah larva; scale bar = 50 μm; region indicated by a square in the photography (B) Undifferentiated gonad of a 40 dah juvenile; scale bar = 20 μm. (C) Juvenile ovary at 58 dah; scale bar = 50 μm. (D) Male testis of 73 dah; scale bar = 50 μm. (E) sox9 mRNA expression in larvae of lambari in different stages of development. Values are expressed as arbitrary units of sox9 mRNA normalized against the expression levels of ef1a amplified from the same template, relative to the expression observed in 26 dah. (F) dmrt1 and sox9 mRNA expression of juvenile lambari. Values are expressed as arbitrary units of both mRNA levels normalized against the expression levels of ef1a amplified from the same template, relative to the expression observed in 58 dah. The asterisk indicates significant difference (p < 0.05) after a Student’s t test comparing the developmental stages indicated. An, anterior region of the larva; Oc, oocyte; Ps, posterior region of the larva; PGC, primordial germ cell; SC, spermatocyte; Se, Sertoli cell; SG, spermatogonia.