Figure 4

Effect of cGMP analogues on maturation of pig denuded oocytes and COCs. (a) Maturation of denuded oocytes cultured in medium supplemented with various concentrations of 8-Br-cGMP or (b) 8-CPT-cGMP for 44 h. (c) Effect of FSH on maturation of COCs in medium supplemented with 1 mM 8-Br-cGMP or (d) 1 mM 8-CPT-cGMP for 44 h. C, control oocytes or COCs cultured in medium without FSH and cGMP analogues; GV, germinal vesicle; GVBD, germinal vesicle breakdown; MII, metaphase II. Asterisks above the column indicate significant differences from the corresponding value in the control group. *P < 0.05; **P < 0.01; ***P < 0.001. Approximately 75 denuded oocytes or COCs were included in each group.