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Table 4 Effects of holding in EH medium at controlled room temperature (25°C) on confocal energy/redox parameters of equine oocytes

From: Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential

Treatment

Nuclear chromatin configuration

n. of analyzed oocytes

Mitochondrial activity

Intracellular ROS levels

Mitochondria/ROS colocalization

EH

FN

3

162.89 ± 101.19

172.93 ± 46.40

§

IMM

2

§

§

§

EH

F/I

4

437.35 ± 298.64

377.13 ± 181.20

0.40 ± 0.32

IMM

9

623.01 ± 376.96a

421.44 ± 164.87

0.38 ± 0.08

EH

CC

20

547.82 ± 499.54

278.53 ± 179.28

0.50 ± 0.10

IMM

19

722.93 ± 390.31a

377.92 ± 184.92

0.49 ± 0.19

EH

PI/MI

0

§

§

§

IMM

3

1292 ± 192.36b

643.67 ± 312.69

0.60 ± 0.10

EH

MII

0

§

§

§

IMM

 

2

§

§

§

  1. Legend: Homogenous/heterogeneously fluorescent nucleus (FN); Fibrillar/Intermediate (F/I); Condensed chromatin (CC); Prometaphase I/Metaphase I (PI/MI); Metaphase II (MII). Mitochondrial activity and intracellular ROS levels are presented as MitoTracker and DCF fluorescence intensities expressed in Arbitrary Densitometric Units (ADU). Mitochondria/ROS colocalization is expressed as Pearson’s correlation coefficient. One-way ANOVA followed by Multiple Comparison Holm-Sidak method: a,b P <0.05; §: values for categories with fewer than 3 oocytes are not shown.