The effect of the traditional Chinese medicine (TCM) on gene expression of the estrogen receptor. MELN cells were treated for 24 hours at 37°C in a 5% CO2 humidified atmosphere with either 10-8M 17β-estradiol (E2) or a 0.3% dilution of the TCM. At the end of the incubation, the mRNA expression levels of five endogenous E2-regulated genes, pS2, the progesterone receptor (PR) , RIP140 , GREB1 , and RARα , were determined by quantitative real-time polymerase reaction. The expression levels of each gene were normalized to that of the housekeeping 28S gene, and quantified in relative units using qBase PLUS. Data are displayed as the average of two determinations ± standard error of the mean. * = p < 0.05 vs ETOH.