Figure 7
The effect of the traditional Chinese medicine (TCM) on gene expression of the estrogen receptor. MELN cells were treated for 24 hours at 37°C in a 5% CO2 humidified atmosphere with either 10-8M 17β-estradiol (E2) or a 0.3% dilution of the TCM. At the end of the incubation, the mRNA expression levels of five endogenous E2-regulated genes, pS2, the progesterone receptor (PR) , RIP140 , GREB1 , and RARα , were determined by quantitative real-time polymerase reaction. The expression levels of each gene were normalized to that of the housekeeping 28S gene, and quantified in relative units using qBase PLUS[26]. Data are displayed as the average of two determinations ± standard error of the mean. * = p < 0.05 vs ETOH.