Figure 3

The dilution-response curves to components B, F, J. The curves were constructed by exposing HELN-ERα (ERα), HELN-ERβ (ERβ), HELN-DA/BERα (DAB-ERα), and HELN-DA/BERβ (DAB-ERβ) cells to increasing dilutions of ingredient B (a), F (b), J (c) of the TCM for 16 hours at 37°C in a 5% CO₂ humidified atmosphere. The results are expressed as a percentage of maximum luciferase activity (100%), which was determined when the cells were exposed to 10^-8M E₂. Each value at each dilution in the response curves is the mean ± standard deviation from three separate experiments. p < 0.05: * = EC₅₀ ERα vs ERβ, ** = Max. luciferase activity ERα vs ∆ A/B ERα, *** = Max luciferase activity ERβ vs ∆ A/B ERβ.