Figure 2

The dilution-response curves to the traditional Chinese medicine (TCM). The curves were constructed by exposing HELN-ERα (ERα), HELN-ERβ (ERβ), HELN-ΔA/BERα (ΔAB-ERα), and HELN-ΔA/BERβ (ΔAB-ERβ) cells to increasing dilutions of the TCM for 16 hours at 37°C in a 5% CO₂ humidified atmosphere. The results are expressed as a percentage of maximum luciferase activity (100%), which was determined when the cells were exposed to 10^-8M E₂. Each value at each dilution in the response curves is the mean ± standard deviation from three separate experiments. p < 0.05: * = EC₅₀ ERα vs ERβ, ** = Max. luciferase activity ERα vs ∆ A/B ERα, *** = Max luciferase activity ERβ vs ∆ A/B ERβ.