Figure 4

Leptin receptor (OB-Rb) protein expression at gestational days (gd) 20 and 50 healthy and arresting conceptus attachment sites. A: Western Blotting images of relative OB-Rb protein expression levels in the endometrial (Endo) and chorioallantoic membrane (CAM) tissues of healthy and arresting attachment sites at gestational day (gd) 20 and gd50. Only the top band (~125 kDa range) was quantified relative to the expression of β-actin (ACTB). B: Histogram represents densitometry values of relative OB-Rb protein expression levels in the Endo and CAM tissues of healthy (white-filled bars) and arresting (black-filled bars) attachment sites at gd20 and gd50. Statistical comparisons were made based on the gd by tissue type interaction. ^abcBars (pulled together from healthy and arresting attachment sites) with different superscripts are significantly different (P < 0.01). The OB-Rb protein production was more pronounced in the endometrium than the CAM, and again more at gd20 than gd50. The lowest OB-Rb production was observed in the gd50 CAM samples compared with the remaining tissues. Histogram bars represent group means plus standard error.