Figure 1

Leptin ( LEP ) mRNA expression level comparison between gestational days (gd) 20 and 50 healthy and arresting conceptus attachment sites. Relative mRNA expression levels of LEP in the endometrium of non-pregnant (NP; grey filled bar), in comparison with endometrial (Endo) and chorioallantoic membrane (CAM) tissues of healthy (white-filled bars) and arresting (black-filled bars) attachment sites at gestational day (gd) 20 and gd50. ^*LEP expression in NP samples was compared with gd20 endometrial tissues only (P < 0.05). Conceptus health did not affect LEP expression. ^aSignificant tissue type by gd interaction existed (P < 0.001). The greatest LEP expression was detected in endometrial samples collected from conceptus attachment sites at gd20 when compared with the remaining tissues on both gestational days. Transcript abundance was quantified by real-time PCR and normalized as a ratio to β-actin (ACTB). Histogram bars represent group means plus standard error.