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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: 1,25-Dihydroxyvitamin D3 increases testosterone-induced 17beta-estradiol secretion and reverses testosterone-reduced connexin 43 in rat granulosa cells

Figure 1

1,25D 3 attenuated testosterone-induced aromatase expression but improved 17β-estradiol secretions. Ovarian granulosa cells from rats were pretreated with 1,25D3 (0.1 μM) for 15 min and subsequently treated with 0.1 μg/mL or 1 μg/mL testosterone (denoted by T) or vehicle (denoted by −) for 24 h. (A) Cells were lysed for protein extraction, and cell lysates were subjected to western blot analysis for aromatase protein detection. The level of aromatase (denoted by Arom) expression was estimated by densitometric analyses after normalization with the β-actin signal (n = 5–6 in each group). (B and C) 17β-Estradiol secretions from the granulosa cells were collected from the medium and assessed over the course of a 24-h period of drug treatments. 17β-Estradiol release data were expressed as the fold-change relative to the vehicle-treated control (n = 4–5 in each group). The values represent the means ± SEM. **, p < 0.005 for comparison with the vehicle-treated group; ##, p < 0.005 for comparison of testosterone/1,25D3 and testosterone alone; a, p < 0.05 for comparison of 1,25D3 alone and vehicle-treated group. The values listed within column indicate the means.

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