Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle

Table 1 Primers used in this study

Primers purpose	Primer name	Primer sequence (5′-3’)
RT-PCR	Syt1-F	AACCCTGTTTTCAATGAGCAA
	Syt1-R	CACTATGTGGGCAAATGCAG
3’-RACE	Syt1-GSP3	GGGCTACAACAGCACTGGAGCGGAG
	Syt1-NGSP3	CTTGCAGCCCGAGGAGGAGGTAGAT
5’-RACE	Syt1-GSP5	CCGTGTTCATCGCAACCTTA
	Syt1-NGSP5	TTCCACCCAGCTCGGAGTAT
RACE	UPM-Long	CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT
	UPM-Short	CTAATACGACTCACTATAGGGC
Real-time PCR	Syt1-S	TATGACAAGATTGGCAAGAAC
	Syt1-A	GGCATCTACCTCCTCCTC
Internal control	18S rRNA-S	CGGACAGGATTGACAGATTGAG
	18S rRNA-A	GCCAGAGTCTCGTTCGTTAT

ISSN: 1477-7827