Relative expression of Syt1 mRNA in the pituitary of Huoyan geese during different stages of the egg-laying cycle. The expression levels of Syt1 were normalized 18S rRNA. The expression levels, calculated by the 2−ΔΔCt method, are presented in arbitrary units (AU). Values are the means ± SEM. The significance of the differences in the levels of expression of Syt1 mRNA was determined using ANOVA followed by Tamhane’s T2 post hoc test. The means marked with the same letter are not significantly different (P < 0.05).