Figure 3

AR occupancies on epididymal AR binding sites associated with androgen-regulated beta-defensins as revealed by ChIP-PCR (A) or ChIP-qPCR (B). A) Input sample (IP) and ChIP samples of anti-AR antibody (AR) or normal IgG (IgG) were analyzed using PCR to confirm AR binding at eleven epididymal target loci. B) ChIP samples were prepared from the caput epididymidis of intact mice (Intact) and mice castrated for 7 days and supplemented with oil (Oil) or testosterone propionate (TP) for an additional 2 days. Enrichment folds were calculated using IgG enrichment as a control. The data are presented as the mean ± SD of three replicates.