Figure 6
From: The term basal plate of the human placenta as a source of functional extravillous trophoblast cells
Cell viability and mRNA expression of term basal plate EVT cells at different times of culture. Cell viability was analyzed by PI incorporation and trypan blue exclusion using complete DMEM/F12 (A) and supplemented IMDM (B). The data represent the mean±SEM of four independent assays. (C) Agarose gel electrophoresis of amplified PCR products of GAPDH, 18S, MMP-2 and MMP-9 genes. (D) Representation of the RT-PCR data as determined by densitometric analysis of gel bands expressed as a ratio of GAPDH expression. The data represent the means±SEM from three independent experiments. **p<0.01; ***p<0.001 versus control.