Cell viability and mRNA expression of term basal plate EVT cells at different times of culture. Cell viability was analyzed by PI incorporation and trypan blue exclusion using complete DMEM/F12 (A) and supplemented IMDM (B). The data represent the mean±SEM of four independent assays. (C) Agarose gel electrophoresis of amplified PCR products of GAPDH, 18S, MMP-2 and MMP-9 genes. (D) Representation of the RT-PCR data as determined by densitometric analysis of gel bands expressed as a ratio of GAPDH expression. The data represent the means±SEM from three independent experiments. **p<0.01; ***p<0.001 versus control.