Table 1 Sequence of primers for real time RT-PCR for TGFβ superfamily members and receptors, CDX2 and NANOG
Gene | Genbank accession number | Primer sequence | Size (bp) |
|---|---|---|---|
BMP2 | BC134682 | F: 5′-AAGGCCCTTGCTTGTCACTTT-3′ | 72 |
|  |  | R: 5′-TGCTTGCCGCTTTTCTCTTC-3′ |  |
BMP3 | XM_587912 | F: 5′-ATCTGTGGCTGAGCTGCTTGT-3′ | 62 |
|  |  | R: 5′-GGAAGGGCTGCCTGAGTCT-3′ |  |
BMP7 | XM_612246 | F: 5′-TGCCACTAGCTCTTCCTGGAA-3′ | 65 |
|  |  | R: 5′-TGAGAGACCCAGGATCCAGAA-3′ |  |
BMP10 | XM_583418 | F: 5′-CGCCCACGAGCAATTCC-3′ | 66 |
|  |  | R: 5′-TCCCCAGGTCCGTTGGA-3′ |  |
SMAD1 | BC116117 | F: 5′-CACCATGAACTGAAACCATTGG-3′ | 68 |
|  |  | R: 5′-GATGCACACCTCCTTCTGCTT-3′ |  |
SMAD5 | DV821574 | F: 5′-GCAACGTTTCCTGATTCTTTCC-3′ | 74 |
|  |  | R: 5′-GGCGGGTAGGGACTATTTGG-3′ |  |
ALK3 | NM_001076800 | F: 5′-TCAGCGAACTATTGCCAAACAG-3′ | 75 |
|  |  | R: 5′-CCCATCCACACTTCTCCGTATC-3′ |  |
ALK6 | NM_001105328 | F: 5′-CCCACCCCTCGTCCAAAG-3′ | 63 |
|  |  | R: 5′-GACCGAGTCTTCTGGACAATGG-3′ |  |
ALK2 | BC133311 | F: 5′-TTGGCCTCATCATTTTGTCTGT-3′ | 70 |
|  |  | R: 5′-CGGAGAGCAACTCCCAATAGG-3′ |  |
ACVR2A | NM_174227 | F: 5′- CCACAAACCCGCCATATCTC -3′ | 185 |
|  |  | R: 5′- TAGCACCCTCTAACACCTCTG -3′ |  |
ACVR2B | NM_174495 | F: 5′-GGAGCCATCAACTTCCAGAG-3′ | 121 |
|  |  | R: 5′-GCATGTACTCATCCACAGGTC-3′ |  |
BMPR2 | XM_002685492 | F: 5′-AACACCACTCAGTCCACCTC-3′ | 120 |
|  |  | R: 5′-GTCAGCATCCTATATCCAAAGCA-3′ |  |
NANOG | NM_001025344 | F: 5′-AAAGTTACGTGTCCTTGCAAACG-3′ | 73 |
|  |  | R: 5′-GAGGAGGGAAGAGGAGAGACAGT-3′ |  |
CDX2 | AM293662 | F: 5′-FCGTCTGGAGCTGGAGAAGGA-3′ | 70 |
|  |  | R: 5′-CGGCCAGTTCGGCTTTC-3′ |  |