Effect of hormones on serum lipid and lipoprotein levels. a) Total serum triglyceride content was significantly increased in E + P (40/6), E + P (100/6), E + P (Menstrual) and E + P (Estrus) groups. b) Free cholesterol levels were increased significantly in mice treated with AI compared to ovariectomized control animals. E + P + T treatment showed highly significant reduction compared to ovariectomized control and AI treatment. Other hormone treatments lowered the free serum cholesterol significantly, except AI + E + P. c) VLDL/LDL cholesterol levels were significantly increased in AI treatment whereas, the combination of various hormone treatments reduced the VLDL/LDL level significantly. d) Serum HDL cholesterol levels were increased significantly in all hormone treated groups compared to AI treatment. Since, all 5, 10 and 15-month time points showed similar trend, 10-month time point was taken as a representative result. All values are expressed as Mean ± SD and p ≤ 0.05 was considered statistically significant. *- represents significant difference between AI treatment and other hormone treatments, #- represents significant difference between ovariectomized control and AI treatment.