Effect of hormones on MCF7-ARO tumor xenograft growth and body weight in female nude mice. a) Mice were treated with AI and hormones according to the experimental setup described in Figure 1 in Methods. Tumor growth was measured twice a week and tumor volume was calculated using the formula 4/3 r12r2, where r1 is the minor radius and r2 is the major radius. Tumor growth was rapid in ovariectomized control and mice received E + P (40/6), whereas E + P + T (100/6/50) and E + P (Menstrual) treatment reduced the tumor growth drastically. b) Body weight of the animals shows toxicity of the treatment and general health of the animals. Ovariectomized control and AI treated mice gained weight constantly throughout the experimental period. E + P (Estrus) and E + P (100/6) group animals showed least weight gain compared to other groups. All values are expressed as mean and for the clarity of the bar-diagram the error bars (representing SD) were removed.