Figure 1
From: Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
Description of the three protocols of bilateral uterine ligation experiments. A. Study scheme of the experiments. After acclimatization, surgery was performed on pregnant mice at E16 (P1), E17 (P2) or E16.5 (P3). Fetuses were extracted before delivery, at E18.5, preceding general observation, weighing, euthanasia and organ collection. B. Ligation localization. In P1, ligation was performed in test animals around the distal part of the uterine vessels, near the cervical end of each uterine horn; in P2 and P3 ligation was performed in test animals around the central part of each uterine horn, generally between fetus 2 and 3. Control animals underwent sham procedure. C. Number of pregnant mice and fetuses (at surgery) used in the three different protocols. P, Protocol.