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Table 2 Primers used for qRT-PCR analysis

From: SOLD1 is expressed in bovine trophoblast cell lines and regulates cell invasiveness

Gene Primer Sequence Position
SOLD1 [NM_001105478] F 5′-GGAAGCACCTGCCAGACTCA-3′ 177–196
R 5′-AAAGCGTGCCATTTTCGAAG-3′ 246–227
TNF-α [NM_174662] F 5′-AAGCCGGTAGCCCACGTT-3′ 297-314
R 5′-TGAGGGCATTGGCATACGA-3′ 375-357
BCL2A1 [NM_001037100] F 5′-TTGCAGATACAGCAACCTGGAT-3′ 73-94
R 5′-GGACAGAGGAAGCCACATCTTG-3′ 145-124
GAPDH [NM_001034034] F 5′-AAGGCCATCACCATCTTCCA-3′ 280–299
R 5′-CCACCACATACTCAGCACCAGCAT-3′ 355–332
  1. SOLD1, secreted protein of Ly-6 domain 1; TNF-α, Tumor necrosis factor α; BCL1A1, Bcl-2-related protein A1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; F, forward; R, reverse; qRT-PCR, quantitative reverse transcription polymerase chain reaction.