Table 2 Primers used for qRT-PCR analysis
From: SOLD1 is expressed in bovine trophoblast cell lines and regulates cell invasiveness
Gene | Primer | Sequence | Position |
|---|---|---|---|
SOLD1 [NM_001105478] | F | 5′-GGAAGCACCTGCCAGACTCA-3′ | 177–196 |
R | 5′-AAAGCGTGCCATTTTCGAAG-3′ | 246–227 | |
TNF-α [NM_174662] | F | 5′-AAGCCGGTAGCCCACGTT-3′ | 297-314 |
R | 5′-TGAGGGCATTGGCATACGA-3′ | 375-357 | |
BCL2A1 [NM_001037100] | F | 5′-TTGCAGATACAGCAACCTGGAT-3′ | 73-94 |
R | 5′-GGACAGAGGAAGCCACATCTTG-3′ | 145-124 | |
GAPDH [NM_001034034] | F | 5′-AAGGCCATCACCATCTTCCA-3′ | 280–299 |
R | 5′-CCACCACATACTCAGCACCAGCAT-3′ | 355–332 |