Skip to main content

Table 2 Studies investigating the relationship between vitamin D status and outcomes in women undergoing ART treatments

From: Vitamin D and assisted reproduction technologies: current concepts

Author, year, [reference #] Country Study design COH Sample size Biological sample(s) used for 25(OH)D assessment Main Outcomes Statistical analysis used for cycle outcome assessment Main finding(s) Main confounding factors considered in the analysis
Potashnik et al., 1992 [47] Israel Prospective cohort Long agonist protocol 10 Serum; follicular fluid (from “one large follicle”) Vitamin D and metabolites’ levels throughout COH Paired t-test 1,25(OH)2D increased at the end of stimulation. No changes observed for 25(OH)D, 24,25(OH)2D None
Ozkan et al., 2010 [38] USA Prospective cohort Long agonist protocol 84 Serum; follicular fluid (pooled from follicles ≥14 mm) CPR Multivariate logistic regression FF levels of 25(OH)D act as positive independent predictor of CPR Age
Race
BMI
Embryos transferred (n)
Anifandis et al., 2010 [39] Greece Retrospective cohort Short agonist protocol 101 Serum; follicular fluid (pooled from all follicles) Glucose levels in follicular fluid; embryo quality; CPR X2-test Higher FF levels of 25(OH)D are associated with lower FF glucose levels and with lower CPR Age
BMI
Oocytes retrieved (n)
Aleyasin et al., 2011 [40] Iran Prospective cohort Long agonist protocol 82 Serum; follicular fluid (pooled from follicles ≥14 mm after oocyte isolation) CPR Multivariate logistic regression No significant association between FF or serum levels of 25(OH)D and CPR Variables showing p value ≤ 0.2 on univariate logistic regression
Rudick et al., 2012 [41] USA Retrospective cohort Long agonist; antagonist; microdose flare protocol 188 Serum CPR Multivariate logistic regression Opposite relation between 25(OH)D levels and IVF outcomes by race: higher levels of 25(OH)D associated with higher CPR in non-Hispanic whites and with lower CPR in Asians Age
BMI
Embryos transferred (n, quality)
Poor ovarian reserve
Firouzabadi et al., 2014 [34] Iran Prospective cohort Long agonist protocol 221 Serum; follicular fluid Chemical pregnancy rate Kruskal-Wallis H test No significant association between FF or serum levels of 25(OH)D and CPR None
Rudick et al., 2014 [42] USA Retrospective cohort Egg donation recipients 99 Serum CPR, LBR Multivariate logistic regression Higher 25(OH)D levels associated with higher CPR and LBR Recipient age
Recipient BMI
Embryos transferred (n, quality)