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Table 1 List of primers and taqman probes used for semi-quantitative RT-PCR

From: Interplacental uterine expression of genes involved in prostaglandin synthesis during canine pregnancy and at induced prepartum luteolysis/abortion

Primer Accession numbers Primer sequence Product lenght (bp)
GAPDH (forward) AB028142 5′-GCT GCCAAATAT GACGACATC A-3′ 75bp
GAPDH (reverse)   5′-GTA GCC CAG GAT GCC TTT GAG-3′  
GAPDH (TaqMan Probe)   5′-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3′  
18SrRNA (forward) FJ797658 5′-GTC GCT CGC TCC TCT CCT ACT-3′ 125bp
18SrRNA (reverse)   5′-GGC TGA CCG GGT TGG TTT-3′  
18SrRNA (TaqMan Probe)   5′-ACA TGC CGA CGG GCG CTG AC-3′  
PGR (forward) NM_001003074 5′-CGA GTC ATT ACC TCA GAA GAT TTG TTT-3′ 113bp
PGR (reverse)   5′-CTT CCA TTG CCC TTT TAA AGA AGA-3′  
PGR (TaqMan Probe)   5′-AAG CAT CAG GCT GTC ATT ATG GTG TCC TAA CTT-3′  
COX2 (forward) HQ110882 5′-GGA GCA TAA CAG AGT GTG TGA TGT G-3′ 87bp
COX2 (reverse)   5′-AAG TAT TAG CCT GCT CGT CTG GAA T-3′  
COX2 (TaqMan Probe)   5′-CGC TCA TCA TCC CAT TCT GGG TGC-3′  
PGFS/AKR1C3 (forward) NM_001012344 5′-AGG GCT TGC CAA GTC TAT TGG-3′ 74bp
PGFS/AKR1C3 (reverse)   5′-GCC TTG GCT TGC TCA GGA T-3′  
PGFS/AKR1C3 (TaqMan Probe)   5′-TCC AAC TTT AAC CGC AGG CAG CTG G-3′  
PGES (forward) NM_001122854 5′-GTC CTG GCG CTG GTG AGT-3′ 89bp
PGES (reverse)   5′-ATG ACA GCC ACC ACG TAC ATC T-3′  
PGES (TaqMan Probe)   5′-TCC CAG CCT TCC TGC TCT GCA GC-3′  
PTGFR (FP) (forward) NM_001048097 5′-ACC AGT CGA ACA TCC TTT GCA-3′ 86bp
PTGFR (FP) (reverse)   5′-GGC CAT CAC ACT GCC TAG AAA-3′  
PTGFR (FP) (TaqMan Probe)   5′-CAT GGT GTT CTC CGG TCT GTG CCC-3′  
PTGER2 (forward) AF075602 5′- CAC CCT GCT GCT GCT TCT C-3′ 78bp
PTGER2 (reverse)   5′-CGG TGC ATG CGG ATG AG-3′  
PTGER2 (TaqMan Probe)   5′-TGC TCG CCT GCA ACT TTC AGC GTC-3′  
PTGER4 (forward) NM_001003054 5′-AAA TCA GCA AAA ACC CAG ACT TG-3′ 96bp
PTGER4 (reverse)   5′-GCA CGG TCT TCC GCA GAA -3′  
PTGER4 (TaqMan Probe)   5′-ATCCGA ATT GCT GCT GTG AAC CCT ATC C-3′  
PGT (forward) NM_001011558 5′-TGC AGC ACT AGG AAT GCT GTT C-3′ 116bp
PGT (reverse)   5′-GGG CGC AGA GAA TCA TGG A-3′  
PGT (TaqMan probe)   5′-TCT GCA AAC CAT TCC CCG CGT G-3′  
HPGD (forward) NM_001284477 5′-GGC AGC GAA TCT CAT GAA CAG-3′ 93bp
HPGD (reverse)   5′-TCT TCT TTC TCA ATG GAT TCA AGGA-3′  
HPGD (TaqMan Probe)   5′-TGA ATG CCA TTT GCC CAG GCT TTG-3′