Table 1 List of primers and taqman probes used for semi-quantitative RT-PCR

GAPDH (forward)

AB028142

5′-GCT GCCAAATAT GACGACATC A-3′

75bp

GAPDH (reverse)

5′-GTA GCC CAG GAT GCC TTT GAG-3′

GAPDH (TaqMan Probe)

5′-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3′

18SrRNA (forward)

FJ797658

5′-GTC GCT CGC TCC TCT CCT ACT-3′

125bp

18SrRNA (reverse)

5′-GGC TGA CCG GGT TGG TTT-3′

18SrRNA (TaqMan Probe)

5′-ACA TGC CGA CGG GCG CTG AC-3′

PGR (forward)

NM_001003074

5′-CGA GTC ATT ACC TCA GAA GAT TTG TTT-3′

113bp

PGR (reverse)

5′-CTT CCA TTG CCC TTT TAA AGA AGA-3′

PGR (TaqMan Probe)

5′-AAG CAT CAG GCT GTC ATT ATG GTG TCC TAA CTT-3′

COX2 (forward)

HQ110882

5′-GGA GCA TAA CAG AGT GTG TGA TGT G-3′

87bp

COX2 (reverse)

5′-AAG TAT TAG CCT GCT CGT CTG GAA T-3′

COX2 (TaqMan Probe)

5′-CGC TCA TCA TCC CAT TCT GGG TGC-3′

PGFS/AKR1C3 (forward)

NM_001012344

5′-AGG GCT TGC CAA GTC TAT TGG-3′

74bp

PGFS/AKR1C3 (reverse)

5′-GCC TTG GCT TGC TCA GGA T-3′

PGFS/AKR1C3 (TaqMan Probe)

5′-TCC AAC TTT AAC CGC AGG CAG CTG G-3′

PGES (forward)

NM_001122854

5′-GTC CTG GCG CTG GTG AGT-3′

89bp

PGES (reverse)

5′-ATG ACA GCC ACC ACG TAC ATC T-3′

PGES (TaqMan Probe)

5′-TCC CAG CCT TCC TGC TCT GCA GC-3′

PTGFR (FP) (forward)

NM_001048097

5′-ACC AGT CGA ACA TCC TTT GCA-3′

86bp

PTGFR (FP) (reverse)

5′-GGC CAT CAC ACT GCC TAG AAA-3′

PTGFR (FP) (TaqMan Probe)

5′-CAT GGT GTT CTC CGG TCT GTG CCC-3′

PTGER2 (forward)

AF075602

5′- CAC CCT GCT GCT GCT TCT C-3′

78bp

PTGER2 (reverse)

5′-CGG TGC ATG CGG ATG AG-3′

PTGER2 (TaqMan Probe)

5′-TGC TCG CCT GCA ACT TTC AGC GTC-3′

PTGER4 (forward)

NM_001003054

5′-AAA TCA GCA AAA ACC CAG ACT TG-3′

96bp

PTGER4 (reverse)

5′-GCA CGG TCT TCC GCA GAA -3′

PTGER4 (TaqMan Probe)

5′-ATCCGA ATT GCT GCT GTG AAC CCT ATC C-3′

PGT (forward)

NM_001011558

5′-TGC AGC ACT AGG AAT GCT GTT C-3′

116bp

PGT (reverse)

5′-GGG CGC AGA GAA TCA TGG A-3′

PGT (TaqMan probe)

5′-TCT GCA AAC CAT TCC CCG CGT G-3′

HPGD (forward)

NM_001284477

5′-GGC AGC GAA TCT CAT GAA CAG-3′

93bp

HPGD (reverse)

5′-TCT TCT TTC TCA ATG GAT TCA AGGA-3′

HPGD (TaqMan Probe)

5′-TGA ATG CCA TTT GCC CAG GCT TTG-3′