Table 2 Primers used for real-time PCR analysis and normalized Ct values in granulosa cells
From: Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare
Gene | Primer forward: 5′-3’ | Primer reverse: 5′-3 | Accession number | Average (Δ) Ct ± SEM | Annealing temperature °C | Fragment size (bp) |
|---|---|---|---|---|---|---|
ACVR1 | CCTCTCCTGTGGGAATGAGG | CTGGAAGCAGCCTTTCTGGT | XM_001491549.3 | 8.95 ± 0.32 | 60 | 100 |
ACVR2B | ATGTACCGGCATCGAAAACC | CGAGCCTTGATCTCCAACAG | XM_001488736.4 | 13.99 ± 0.39 | 60 | 119 |
TGFBR2 | GACCCCAAGCTCACCTACCA | TGCACTCATCAGAGCTACAGGA | XM_005600872.1 | 0.8 ± 0.43 | 60 | 124 |
CDKN2B | CCGAGCTGCTACTGCTCCAC | CACCAGCGTGTCCAGGAAG | XM_001496235.3 | 13.93 ± 0.70 | 60 | 107 |
COL1A2 | AGGTTTCCAAGGACCTGCTG | GGTTTTCCAGGGTGACCATC | XM_005609220.1 | 16.21 ± 0.75 | 60 | 117 |
COL3A1 | GTCCCAACCCAGAGATTCCA | CGCTACTTTCATTTCCTTTCAGG | XM_001917620.3 | 2.05 ± 0.57 | 60 | 102 |
USF2 | GGATACCACGGCTGTGTCAG | ATCGTCCTCTGCGTTCCTGT | XM_005596152.1 | 4.23 ± 0.70 | 60 | 114 |
IL6 | GGCAGAAAAAGACGGATGCT | CACCCTTGAACTCGTTCTGGA | XM_005609172.1 | 13.03 ± 0.38 | 60 | 122 |
ITGB7 | TGCCGAAGGATACCCTGTAGA | CTGCAGCTTCTCCAGCAAGG | XM_005611222.1 | 14.58 ± 0.82 | 60 | 112 |
TGFB1 | CTCAGTGCCCACTGCTCCT | CATCAATGGTGGCCAGATCA | XM_005596086.1 | 2.68 ± 0.27 | 60 | 100 |
ID1 | ACATGAACGGCTGCTACTCG | TCCAACTCCAGGTCCCAGAT | XM_005604549.1 | 10.02 ± 0.51 | 60 | 124 |
ID2 | CATCCCCCAGAACAAGAAGG | TGGTGATGCAGGCTGACAAT | XM_001503611.3 | 2.37 ± 0.18 | 60 | 180 |
ACTB | CGACATCCGTAAGGACCTGT | CAGGGCTGTGATCTCCTTCT | NM_001081838.1| | 60 | 99 | |
GAPDH | AGAAGGAGAAAGGCCCTCAG | GGAAACTGTGGAGGTCAGGA | NM_001163856.1 | 60 | 87 |