Table 1 Antibodies utilized for Western blot analysis
From: Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare
Protein target | Antibody | Catalog number | Accession number | Species raised | Dilution used |
|---|---|---|---|---|---|
ACVR1 | Antibody raised against a peptide mapping to the N-terminus of ACVR1 of human origin | sc-5671 | Q04771 | Goat polyclonal | 0.4 μg/ml |
Horseradish peroxidase conjugated anti-goat secondary antibody | sc-2020 | 0.2 μg/ml | |||
ACTB | Antibody raised against ACTB of avian origin | sc-47778 | P60709 | Mouse monoclonal | 0.1 μg/ml |
Horseradish peroxidase conjugated anti-mouse secondary antibody | ab-6789 | 1 μg/ml |