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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare

Figure 4

Levels of ACVR1 mRNA and protein and ID2 mRNA in granulosa cells following exosomal treatment. (A) Relative levels of ACVR1 and ID2 in granulosa cells following treatment with exosomes isolated from follicular fluid of mid-estrous and pre-ovulatory follicles. Values on y-axis indicate 1/normalized (Δ) Ct values and relative to geometric mean of ACTB and GAPDH. Different letters indicate P < 0.05 compared to control (no exosome treatment). (B) ACVR1 protein level in pre-ovulatory granulosa cells following treatments with follicular fluid exosomes. Replicates are composed of granulosa cells from pre-ovulatory follicles (n = 4) and exosomes isolated from follicular fluid of pre-ovulatory (n = 4) and mid-estrous follicles (n = 4). Bottom panels indicates the Western blot images with (upper band) ACVR1 and (lower band) ACTB.

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