Skip to main content
Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Use of antagonists and morpholinos in loss-of-function analyses: estrogen receptor ESR2a mediates the effects of 17alpha-ethinylestradiol on primordial germ cell distribution in zebrafish

Figure 1

Fluorescence images of zebrafish PGCs after exposure to various concentrations of EE2. A: In all treatment groups, normal PGCs were observed in the anterior region of the yolk extension at 24 hpf. B: In the 1 ng/L, 10 ng/L, and 100 ng/L EE2 exposure groups, ectopic PGCs were primarily observed along the branchial arch, similarly to the spadetail mutant. C: In the 500 ng/L, 1 μg/L and 2 μg/L EE2 exposure groups, many ectopic PGCs were observed along the branchial arch, on the back, in the abdomen, and along the trunk. A’, B’, and C’: Bright-field views show the morphology of the embryos.

Back to article page

Reproductive Biology and Endocrinology

ISSN: 1477-7827

Contact us