Figure 9
Effects of IGF1 on B6 embryo development in 5% O 2 . B6 zygotes were cultured in groups of 10 μL KSOM media plus amino acid and Paf and supplemented with a range of doses of mouse recombinant IGF1. (A) The proportion of zygotes forming blastocysts were assessed 108 h post-hCG. There was an overall adverse dose-dependent effect IGF1 dose on the rate of blastocysts formation P < 0.001, and ** higher dose significant fewer cells (p < 0.01) compared to controls (B) total number of cells per blastocyst formed. There was an overall adverse dose-dependent effect of IGF1 (p < 0.001) and multiple comparison of means showed * 1 ng dose to have caused significantly more (p < 0.01) and ** higher dose significant fewer cells (p < 0.01) compared to controls. The results were representative of the three independent replicates with at least 80 embryos each dose. (C) The blastocyst rate of B6 zygotes cultured communally in KSOM media plus amino acid and Paf at 5% O2 with or without 1 ng/mL IGF1 was shown, and the cell number was compared with blastocysts collected directly from the uterus 96 h post-hCG. *p < 0.001, compared to the fresh embryos. **p < 0.01, compared to the culture conditions without IGF1. The results were representative of three independent replicates and each treatment had at least 90 embryos.