Figure 7
The effects of exogenous Paf on growth rates. Hybrid strain and B6 mouse zygotes were cultured individually in a drop of 10 μL sIVF media with (Paf) or without (Control) supplementation with 37.2 nM Paf under 5% O2. (A) the rates of the blastocyst formation at 96 and 116 h post-hCG, and the rate of blastocyst hatching, (B) the number of total cells in each blastocyst and (C) the number of the cells with fragmented nuclei were analysed. Each culture group had at least 60 hybrid strain embryos and 50 B6 embryos. Statistically significant effects are shown on the graphs. DENSITY and STRAIN were the dependent variables, and INT (abbreviation of interaction) was the interaction effect between DENSITY and STRAIN.