Figure 3

The expression of BMP15 protein in FF. The comparison of expression of BMP15 protein (A) in follicular fluid derived from non-stimulated gilts with natural estrus (group I; n = 8), stimulated with PMSG/hCG (group II; n = 5) or with PMSG/hCG + PGF2α (group III; n = 7). Representative bands of western blots are presented (B). The bands represent three forms of BMP15 found in FF: premature protein (band 1, ~65 kDa), cleaved proregion (band 2, ~50 kDa) and mature protein (band 3, ~25 kDa). Data are expressed in arbitrary optical density units as the ls mean ± S.E.M. Different superscripts depict significant statistical differences at p < 0.05.