DNA methylation in spermatozoa of OHFD analyzed by BS. DNA methylation of paternally imprinted gene H19 and maternally imprinted gene Peg3 was further analyzed by BS. All the samples were pooled together for each group. (A) represents H19 methylation status; the number shows the methylation%; (B) shows the methylation patterns of Peg3. Black circle, methylated; white circle, unmethylated; blank loci, CpG lost.