Leptin receptor signaling inhibits ovarian follicle development and egg laying in chicken hens

Lei, Ming M; Wu, Si Q; Li, Xiao W; Wang, Cong L; Chen, Zhe; Shi, Zhen D

doi:10.1186/1477-7827-12-25

Reproductive Biology and Endocrinology

Table 1 Primers used in the real-time quantitative PCR of genes in chicken samples

From: Leptin receptor signaling inhibits ovarian follicle development and egg laying in chicken hens

Gene	Accession number	Primer sequences (5′-3′)	Length(bp)
β-actin	L08165	upstream: CCGAGAGAGAAATTGTGCGTGAC	166
β-actin	L08165	downstream: TCGGGGCACCTGAACCTCTC	166
FSHβ	AY029204	upstream: GGCTGCGGTGACCATCCTGAATC	101
FSHβ	AY029204	downstream: GGCCCCAGTCCTCTCACAGTGCA	101
GnRH-I	JN609557	upstream:TGTCCTCCTGTTCACCGCATCTG,	222
GnRH-I	JN609557	downstream: TCGATCAGGCTTGCCATGGTTTC	222
LHβ	HQ872606	upstream:GGGGGGAGCGCAGGTGTTG	220
LHβ	HQ872606	downstream: CCCGCAGGCCGTGGTGGT	220
AgRP	AB029443	upstream: TCCCCTCGCCGCTGTGTC	137
AgRP	AB029443	downstream: CATGGGAAGGTGGTGCTGATC	137
NPY	NM_205473	upstream: GGAAAGAGATCAAGCCCAGAGAC	193
NPY	NM_205473	downstream: ATGCACTGGGAATGACGCTATG	193
orexin	AB056748	upstream: CGCTGGGCAAGAGGAAGAG	117
orexin	AB056748	downstream: GGCGCTCCTCACGTTTGC	117
MC4R	AY545056	upstream: GCCAAGAACAAGAACCTCCATT	150
MC4R	AY545056	downstream: TATGGTAAAGCTCTGTGCGTCTG	150
POMC	NM_001031098	upstream: GGCCGAGGCACCCGTGTAC	141
POMC	NM_001031098	downstream: GCGGGGTGGTGGGGTGAC	141
LEPR	NM_204323	upstream: TTTGCTGTTGGGCTTTCTTCAC	148
LEPR	NM_204323	downstream: AACCAGACCGGCTCCGTACA	148
FSHR	NM_205079	upstream: TCCCACCAATGCCACAGAAC	155
FSHR	NM_205079	downstream: TGGGAAGGCTGGAAAACACA	155
LHR	AB009283	upstream: GGGCATGAGCAACGAATCG	124
LHR	AB009283	downstream: CCGCCTGAGGTTTTTGTTGTC	124
IGF-I	NM_001004384	upstream: TGCTGCTTTTGTGATTTCTTGAA	138
IGF-I	NM_001004384	downstream: AACCAGCTCAGCACCACACAGT	138
fas	NM_001199487	upstream: TTCCCACACACACTGCACATAA	153
fas	NM_001199487	downstream: CACACCGAGAAGAATTGCAGTAA	153
caspase3	NM_204725	upstream: CCACGCTCAGGGGAAGATGTAT	173
caspase3	NM_204725	downstream: CGGTATCTCGGTGGAAGTTCTTA
bcl2	NM_205339	upstream: CGCCGCTACCAGAGGGACTTC	192
bcl2	NM_205339	downstream: CGCCGCCGAACTCGAAGAAG	192
StAR	NM_204686	upstream: GCCGGACGTGGGTAAGGTGT	184
StAR	NM_204686	downstream: CGCCGTCTCGTGGGTGATC	184
CYP19A1	NM_001001761	upstream: TGCCAGTTGCCACAGTGCCTATC	112
CYP19A1	NM_001001761	downstream: GGCCCAATTCCCATGCAGTATC	112
CYP17A1	NM_001001756	upstream: CGGGCAGCTTTCAGGCATG	189
CYP17A1	NM_001001756	downstream: TGGCCATGATGTTGTGCACGTT	189
PPAR	AB045597	upstream: GCTCCAGGATTGCCAAAGTG	137
		downstream: TCCCCACACACACGACATTCA

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ISSN: 1477-7827

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