Figure 3

Ovarian expression of VEGF, its receptor VEGFR-2, and TSP-1 in CD36−/− and WT mice. A. Ovaries from CD36−/− and WT mice were immunostained for VEGF, VEGFR-2 and TSP-1. B. Immunohistochemical analysis showed that ovaries from CD36−/− mice had higher (p < 0.05) percentage positive ovarian tissue for VEGF and VEGFR-2 compared to WT controls. The percentage of tissue immunopositive for TSP-1 was not different between groups. C. Western blot analysis was performed on tissue lysates from WT and CD36−/− mice. D. There was a significant (* - p < 0.05) increase in VEGF and VEGFR-2 protein in CD36−/− ovaries compared to WT. No changes were observed in TSP-1 protein levels between groups.