Figure 3

Twenty-five serial optical sections analysis of bioenergy/redox parameters in a dromedary camel oocyte. Confocal microscopy twenty-five optical plane analysis of bioenergy/oxidative status in a dromedary camel MII stage oocyte representative of the PN/PCTN mitochondrial pattern. Concerning mt distribution (A), in planes nearer the equatorial position (planes 6–17), the perinuclear/pericortical tubular network (PN/PCTN) pattern can be observed. The section circled in white indicates the equatorial plane which was used for quantification analysis as reported in Figure 1. Values of mitochondria activity (A), intracellular ROS level (B) and mt/ROS colocalization (C) of the individual oocyte shown in this figure are: 1183 ADU, 1728 ADU and 0.54 Pearson’s coefficient, respectively. Scale bar represents 60 μm.