Figure 1

Quantitative analysis of bioenergy/redox parameters in dromedary camel oocytes before and after in vitro maturation. Mean ± SD of mitochondrial (mt) activity (Panel A), intracellular ROS levels (Panel B) and mt-ROS colocalization (Panel C) of dromedary camel oocytes, as examined before and after in vitro maturation. Numbers of analyzed oocytes per group are indicated above the histograms. Mitochondrial activity and ROS levels are expressed as MitoTracker Orange CMTM Ros fluorescence intensity and 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCF-DA) fluorescence intensity in Arbitrary Densitometric Units (ADU). Mt/ROS colocalization is expressed as Pearson’s correlation coefficient of MitoTracker Orange CMTM Ros and DCF fluorescent labelling. One-way ANOVA: a,b: P < 0.001; a,c: P < 0.01; a,d: P < 0.05.