Development of testes was recovered partly by decreasing leptin concentration by the withdrawal of MSG treatment. In addition to the alterations in Figure 4, some other parameters were influenced by MSG treatment. Seminiferous tubules and Leydig cells (A), diameter of seminiferous tubules (B), number of seminiferous tubules (C), number of spermatogonia (SG), spermatocytes (SC), sperm (D) and leydig cells (E) were analysed as described above. The means were compared using a one-way analysis of variance followed by Student-Newman-Keuls test to conduct multiple comparisons. *, p < 0.05 compared with the 56MSG-treated mice group; #, p < 0.05 compared with the 56NS-treated mice group; NSD, non-significant difference.