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Table 1 Primer sequences used in the qPCR experiments

From: High-fat diet decreases the expression of Kiss1 mRNA and kisspeptin in the ovary, and increases ovulatory dysfunction in postpubertal female rats

Gene name

Primer sequences (5’–3’)

Expected size(bp)

Accession Number (GenBank)

Kiss1

Forward TGCTGCTTCTCCTCTGTGTGG

110

NM_181692.1

 

Reverse ATTAACGAGTTCCTGGGGTCC

  

Kiss1r

Forward CTTTCCTTCTGTGCTGCGTA

102

NM_023992.1

 

Reverse CCTGCTGGATGTAGTTGACG

  

GDF9

Forward GAGGAAACACAGGTGGATTGAGATG

174

NM_021672.1

 

Reverse AGATACAAGATGAGCGAGGGCG

  

BMP15

Forward GCTAAAATGGTGAGGCTGATAAA

180

NM_021670.1

 

Reverse ATGGCAGGAGAGATGGTAATG

  

FSHR

Forward CTACACATTGACAGCCATCACCCTA

121

NM_199237.1

 

Reverse GGCAAAAGTCCAGCCCAATACC

  

PTGS2

Forward TCTCCAACCTCTCCTACTACACCA

139

NM_017232.3

 

Reverse ATGAACTCTCTCCTCAGAAGAACCT

  

HPRT1

Forward CCCCAAAATGGTTAAGGTTGC

175

NM_012583.2

 

Reverse TCCACTTTCGCTGATGACACAA

  
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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