Effects of exposure to an HFD on kisspeptin-IR in the theca cells of preovulatory follicles. At the prooestrus stage, kisspeptin-IR was detected in the theca cells (arrows) of preovulatory follicles from NCD controls (A). The level of kisspeptin-IR in the theca cells of preovulatory follicles was significantly lower in the HFD-fed rats (B). (C) A higher magnification of the boxed area in (B). Immunostaining was absent in sections from negative control rats (D). The MOD for kisspeptin in the theca cells are summarised in (E) and (F). GC, granulosa cell; PC, preovulatory follicle; TC, theca cell. Photomicrographs illustrate the prooestrus stage of the oestrous cycle at PND 42. Scale bar = 50 μm. *P < 0.05, versus NCD controls (n = 4–6 for each group).