Figure 6

Effects of exposure to a HFD on kisspeptin-IR in the theca cells of antral follicles. Kisspeptin-IR was detected in the theca cells (arrows) of antral follicles from the NCD controls (A). A representative section shows that the level of kisspeptin-IR in the theca cells of the antral follicles was significantly lower in the HFD-fed rats (B). (C) A higher magnification of the boxed area in (B). Immunostaining was absent in sections from the negative controls (D). The MOD for kisspeptin in the theca cells are summarised in (E) and (F). AC, antral follicle; GC, granulosa cell; TC, theca cell. Photomicrographs illustrate the prooestrus stage of oestrous cycle at PND 42. Scale bar = 50 μm. *P < 0.05. n = 4–6 for each group.