Table 2 Real–time PCR conditions

1

Pre-incubation

95°C

5 min

2

Denaturation

95°C

20 sec

3

Annealing

60°C

15 sec

4

Extension

72°C

15 sec

5

Melting

95°C

5 sec

70°C

60 sec

97°C

1 s (continuous acquisition)