Skip to main content

Table 2 Real–time PCR conditions

From: Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density

Step Description Temperature Time
1 Pre-incubation 95°C 5 min
2 Denaturation 95°C 20 sec
3 Annealing 60°C 15 sec
4 Extension 72°C 15 sec
5 Melting 95°C 5 sec
70°C 60 sec
97°C 1 s (continuous acquisition)