Table 1 Primers sequences used for PCR amplification and conditions
Gene | Sequence | Tm (°C) | Cycles | Product size (bp) |
|---|---|---|---|---|
Oct-4 | FW: AGCACGAGTGGAAAGCAACTC | 57 | 38 | 497 |
RV: CAAGCTGATTGGCGATGTGAG | ||||
Sox-2 | FW: ACGCTCATGAAGAAGGATAA | 58 | 42 | 330 |
RV: GTAGGACATGCTGTAGGTGG | ||||
Nanog | FW: TACCTCAGCCTCCAGCAGAT | 56 | 42 | 460 |
RV: CACCTCCAA ATCACTGGCAG | ||||
c-Kit | FW: GTCATTGTTGGCTACGAGAT | 57 | 28 | 142 |
RV: AACACGAGGTCATCCACTAT | ||||
GDF-9 | FW: TTGCTGTTGCCTGTAGATGG | 57 | 28 | 204 |
RV: GAAGAGCCGGACGGTATTGT | ||||
VASA | FW: GCTCAA ACAGGGTCTGGGAAG | 58 | 35 | 145 |
RW: GGTTGATCAGTTCTCGAGTTC | ||||
GAPDH | FW: ACCACAGTCCATGCCATCAC | 57 | 25 | 452 |
RV: TCCACCACCCTGTTGCTGTA |