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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Quantitative bioluminescence imaging of transgene expression in intact porcine antral follicles in vitro

Figure 3

Luciferase activities in isolated granulosa cells from intact follicles in DNA only, 1 μg, 2 μg, and 3 μg groups. Luciferase activities in isolated granulosa cells from intact follicles in DNA only, 1 μg, 2 μg, and 3 μg groups. To demonstrate luciferase activity in follicular granulosa cells, luciferase activity of isolated granulosa cells (2 × 105 cells per well) from intact follicles in the DNA only (n = 9), 1 μg (n = 8), 2 μg (n = 8), and 3 μg (n = 7) groups were quantified using standard luciferase assay (Bright-Glo) in lysed cells (A). The data were expressed as mean ± SEM of Luciferase Activity (Photons Flux/2 × 105 cells). Values with different superscripts in a group are significantly different (P < 0.05).Luminescence imaging of luciferase activity with medium binning in a 24-well plate format (B). The scale bar on the right shows pseudocolor display for the photon flux of the luciferase with red and blue representing highest and lowest values, respectively. The gray scale photographic image and bioluminescence color image were superimposed.

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