Figure 2

Time courses of bioluminescence emitted from transfected intact antral follicles with increasing doses of luciferase reporter gene. A total of n = 87 follicles were randomly distributed in four different groups (DNA only, 1 μg, 2 μg, and 3 μg groups). DNA only group represents a group of intact follicles (n = 22) transfected with 2 μg pGL4 (a luciferase reporter gene) only; 1 μg group represents a group of intact follicles (n = 22) transfected with 1 μg of pGL4: lipid complexes; 2 μg group represents a group of intact follicles (n = 21) transfected with 2 μg of pGL4: lipid complexes; and 3 μg group represents a group of intact follicles (n = 22) transfected with 3 μg of DNA:lipid complex. After 20 hours transfection, each follicle was imaged with series of 30 sec exposure time and 5 binning up to 10 minutes. Data are plotted as mean ± SEM of Luciferase Activity (Photon Flux; p/s) over time. Values with different superscripts in a group are significantly different (P < 0.05). *indicates significant difference (P < 0.05) compared to each other.