Figure 6

The effects of OSM and STAT3 inhibitors on in vitro trophoblast proliferation. OSM (20 ng/mL) did not induce significant increase in cell proliferation after 24 h of culture (A), but induced a significant increase in cell proliferation after 48 h of culture to 2.1-fold (*p < 0.01) of the control (B). Cell proliferation was significantly decreased with stattic (1 μM) treatment, compared to the control (⁺p < 0.05), at 48 h experiment (B), not at 12 h experiment (A). There was no significant difference in cell proliferation between the results of the co-treatment of OSM with stattic and the control (A and B). Data shown are means ± SEM of 3 independent experiments. The absorbance of samples was measured using a 96-well plate reader at 450 nm. The reference wavelength was 650 nm.