Figure 5

Effects of STAT3 inhibition on OSM-induced migrations in HTR8/SVneo cells. Cells were incubated for 12 h at 37°C in 5% CO₂ with or without OSM (20 ng/mL), or with or without pretreatment with stattic (1 μM) for 1 h. Cell wounding assays were conducted and assessed using an LEICA DM IRB/DC 300 microscope (Germany) at 100× magnification. Cell migration distance was measured using Olympus 6.51 software and compared with baseline measurements. Three independent experiments were performed. A representative experiment from these experiments is shown in (A). Results were expressed as a percentage of the control (means ± SEM) (B) compared to the control, *p < 0.05.