Indirect immunofluorescence staining of E-cadherin. Compared with unstimulated cells (A), incubation of HTR8/SVneo cells in the presence of 20 ng/mL OSM for 48 h resulted in a robust downregulation of E-cadherin (B), whereas there was no significant expression change after pretreatment with stattic (1 μM, 1 h) (C). The expression of E-cadherin after pretreatment with stattic and 48 h incubation with OSM was similar to the expression of E-cadherin in unstimulated cells (D). Results shown are representative of 3 independent experiments. Magnification, 1000×. Cells were counterstained with DAPI. Scale bar, 20 μm; differential interference contrast scale bars, 10 μm.