Effects of OSM (20 ng/mL) on mRNA and protein expression of E-cadherin in extravillous cytotrophoblast cells (HTR8/SVneo). Total RNA was extracted from the cells, with or without OSM stimulation for 48 h, and mRNA levels were measured by real-time quantitative RT-PCR analysis (A). E-cadherin mRNA levels were standardized to GAPDH mRNA levels. Results are presented as means ± SEM of 3 independent experiments. The representative effects of OSM on E-cadherin as observed from the western blots are shown in relation to the control (48 h) (B). Quantitative results (C) of western blots were normalized to GAPDH and presented as a fold change over the controls (untreated = 1). Data shown are the means ± SEM of 3 independent experiments compared to the control (*p < 0.05).